Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 116, Issue 50, Pages 25278-25286Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1911262116
Keywords
S-layers; electron cryotomography; Sulfolobus; archaea; subtomogram averaging
Categories
Funding
- Max Planck Society
- University of Exeter Research Fellow's Startup grant
- European Research Council (ERC) starting grant MICROROBOTS
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Surface protein layers (S-layers) often form the only structural component of the archaeal cell wall and are therefore important for cell survival. S-layers have a plethora of cellular functions including maintenance of cell shape, osmotic, and mechanical stability, the formation of a semipermeable protective barrier around the cell, and cell-cell interaction, as well as surface adhesion. Despite the central importance of S-layers for archaeal life, their 3-dimensional (3D) architecture is still poorly understood. Here we present detailed 3D electron cryomicroscopy maps of archaeal S-layers from 3 different Sulfolobus strains. We were able to pinpoint the positions and determine the structure of the 2 subunits SlaA and SlaB. We also present a model describing the assembly of the mature S-layer.
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