Journal
BIORESOURCE TECHNOLOGY
Volume 216, Issue -, Pages 203-210Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.05.077
Keywords
Laccase; Immobilization; Core-shell magnetic copper alginate beads; Water pollutants; Real wastewater
Funding
- National Research Foundation of Korea (NRF) - Korea government (MEST) [2011-0028723]
- The GAIA Project by Korea Ministry of Environment [RE201402059]
- Gyeongsang National University
- National Research Foundation of Korea [22A20130012323, 2011-0028723] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Immobilization of laccase has been highlighted to enhance their stability and reusability in bioremediation. In this study, we provide a novel immobilization technique that is very suitable to real wastewater treatment. A perfect core-shell system composing copper alginate for the immobilization of laccase (Lac-beads) was produced. Additionally, nFe(2)O(3) was incorporated for the bead recycling through magnetic force. The beads were proven to immobilize 85.5% of total laccase treated and also to be structurally stable in water, acetate buffer, and real wastewater. To test the Lac-beads reactivity, triclosan (TCS) and Remazol Brilliant Blue R (RBBR) were employed. The Lac-beads showed a high percentage of TCS removal (89.6%) after 8 h and RBBR decolonization at a range from 54.2% to 75.8% after 4 h. Remarkably, the pollutants removal efficacy of the Lac-beads was significantly maintained in real wastewater with the bead recyclability, whereas that of the corresponding free laccase was severely deteriorated. (C) 2016 Elsevier Ltd. All rights reserved.
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