Journal
BIORESOURCE TECHNOLOGY
Volume 219, Issue -, Pages 559-565Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.08.010
Keywords
Phaeodactylum tricornutum; Oxidative stress; Nitrogen limitation; Lipid accumulation; Hydrogen peroxide
Funding
- National Science Foundation Chemical, Bioengineering, Environmental, and Transport Systems (NSF - CBET) Award [1512250]
- UC Davis Biochemistry, Molecular, Cellular, and Developmental Biology Graduate Group
- Directorate For Engineering [1512250] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys [1512250] Funding Source: National Science Foundation
Ask authors/readers for more resources
Exogenous application of dilute hydrogen peroxide (H2O2) increases neutral lipid production in Phaeodactylum tricornutum. Exposing early stationary phase cultures of P. tricornutum to 0.25-2 mM H2O2 increases the amount of neutral lipids per biomass (mg/mg) by > 100% at 24 h post H2O2 treatment as determined upon lipid extraction and analysis using a neutral lipid assay. H2O2 treatment increased the total levels of neutral lipids harvested up to 50%, from 64 mg/L to 96 mg/L, demonstrating its possible effectiveness as a pre-harvest strategy to enhance the biofuel feedstock potential of P. tricornutum. The effects of H2O2 on biomass are concentration dependent; increasing concentrations of H2O2 reduce the levels of isolated biomass. Analysis of combined stressors demonstrates that H2O2 treatment exhibits synergistic effects to enhance neutral lipid production under nitrogen-depleted, but not phosphorus-depleted conditions, suggesting that the effects of hydrogen peroxide on lipid production are influenced by environmental nitrogen levels. (C) 2016 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available