Journal
BIORESOURCE TECHNOLOGY
Volume 218, Issue -, Pages 962-968Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.07.023
Keywords
Autotrophic nitrogen removal; Dissolved oxygen; Microenvironment; Bacterial community
Funding
- National Natural Science Foundation of China [51278508]
- National Science and Technology Major Project for Water Pollution Control and Remediation of China [2012ZX07307-002]
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The performance of four identical sequencing biofilm batch reactors (SBBR) for autotrophic nitrogen removal was investigated with 2000 mg/L ammonia-containing mature landfill leachate at 30 degrees C. The main objective of this study was to evaluate the effects of dissolved oxygen (DO) on the performance and microbial community of single-stage nitrogen removal using anammox and partial nitritation (SNAP) system. At an applied load of 0.5 kg N m (3) d (1), average total nitrogen removal efficiency (TNRE) above 90% was long-term achieved with an optimal DO concentration of 2.7 mg/L. The microelectrode-measured profiles showed the microenvironments inside the biofilms. 16S ribosomal Ribonucleic Acid (rRNA) amplicon pyrosequencing and denaturing gradient gel electrophoresis (DGGE) were used to analyze the microbial variations of different DO concentrations and different positions inside one reactor. (C) 2016 Elsevier Ltd. All rights reserved.
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