4.6 Article

Infrared spectroscopy of serum as a potential diagnostic screening approach for naturally occurring canine osteoarthritis associated with cranial cruciate ligament rupture

Journal

OSTEOARTHRITIS AND CARTILAGE
Volume 28, Issue 2, Pages 231-238

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2019.10.006

Keywords

Osteoarthritis; Biomarker; Infrared spectroscopy; Stifle; dog; serum

Funding

  1. Canadian Institutes of Health Research Grant-Regional partnership fund - Innovation PEI [97027]
  2. Companion Animal Trust Fund - University of Prince Edward Island
  3. Cohn Family Chair for Small Animals - Oklahoma State University
  4. Boehringer Ingelheim financial incentive for client-owned dog recruitment

Ask authors/readers for more resources

Objective: To evaluate infrared (IR) spectroscopy of serum as a screening tool to differentiate dogs affected by naturally occurring osteoarthritis (OA) associated with cranial cruciate ligament rupture (CrCLR) and controls. Method: 104 adult dogs with CrCLR (affected group) and 50 adult control dogs were recruited for this prospective observational study. Serum samples were collected preoperatively from CrCLR dogs and from a subset of these dogs at 4-, and 12-week post-surgical intervention to stabilize the affected stifles. Serum was collected once from control dogs. Dry films were made from serum samples, and IR absorbance spectra acquired. Data preprocessing, principal component analysis and multivariate analysis of covariance were performed to separate samples from the two groups, and to evaluate temporal differences. Weighted logistic regression with L1 regularization method was used to develop a predictive model. Model performance based on an independent test set was evaluated. Results: Spectral data analysis revealed significant separation between the sera of CrCLR and control dogs (P < 0.0001), but not amongst different time points in the OA group. The sensitivity, specificity, AUC and accuracy of the test set were 84.62%, 96.15%, 93.20% and 92.31% respectively. Conclusions: These findings confirm the potential of IR-spectroscopy of serum with chemometrics methods to differentiate controls from dogs with OA associated with CrCLR. This is the first step in development of an economic, and comparatively simple IR-based screening serum test for OA. Utility of this tool as a clinical screening and diagnostic test requires further investigation and validation. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available