4.8 Article

Excitation of One Fluorescent Probe at Two Different Wavelengths to Determine the Concentration and Enantiomeric Composition of Amino Acids

Journal

ORGANIC LETTERS
Volume 21, Issue 22, Pages 9036-9039

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.orglett.9b03437

Keywords

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Funding

  1. Sichuan Science and Technology Program [2019JDTD0016]
  2. State Scholarship Fund from China Scholarship Council [201708510004]
  3. US National Science Foundation [CHE-1565627]

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When a novel fluorescent probe made of BINOL and coumarin is excited at lambda(1) = 365 nm in a neutral buffer solution, it shows fluorescence enhancement at 465 nm in the presence of an amino acid with a very small difference between the two enantiomers. When excited at lambda(2) = 467 nm, it shows highly enantioselective fluorescence enhancement at 534 nm. This allows the determination of both concentration and enantiomeric composition of amino acids.

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