Journal
OPTICS LETTERS
Volume 44, Issue 21, Pages 5238-5241Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.44.005238
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Funding
- Research Grants Council, University Grants Committee [CityU T42-103/16-N, E-HKU701/17, HKU 17200219, HKU 17209018, HKU C7047-16G]
- National Natural Science Foundation of China [61631166003, 61675081, N_HKU712/16]
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We demonstrate dual-Airy-beam-scanning-based volumetric two-photon microscopy (TPM) with depth-resolving capability. A pair of Airy beams with opposite acceleration is used as the excitation lights to sequentially illuminate the sample, and depth information can be resolved based on the deflection of the Airy beam. The depth-resolving range of the volumetric TPM is up to 32 mu m. The advantages of the depth-resolved volumetric TPM are the depth-resolving capability over Bessel-beam-based TPM and less scanning times over traditional Gaussian-beam-based TPM. The depth-resolved volumetric TPM provides a promising fast imaging tool to study the dynamics in neural biology. (C) 2019 Optical Society of America
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