Journal
BIORESOURCE TECHNOLOGY
Volume 218, Issue -, Pages 373-379Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.06.088
Keywords
Crude glycerol; Impurities; Lipid production; Rhodosporidium toruloides yeast 32489
Funding
- National Environmental Protection Public Welfare Science and Technology Research Program of China [201309023]
- Beijing Natural Science Foundation [3144036]
- [2013DFG92600]
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Crude glycerol (byproduct of biodiesel preparation) was utilised as the carbon source to produce lipid using oleaginous yeast Rhodosporidium toruloides 32489. Under the same conditions, lipid production with crude glycerol was higher than those produced with glucose and pure glycerol. The effects of 4 main impurities in crude glycerol (methyl oleate, sodium oleate, NaCl and methanol) on lipid production were investigated. Compared with utilising pure glycerol, addition of methyl oleate, sodium oleate, and NaCl impurities increased lipid production by 47.0%, 68.0% and 64.0%, respectively, while methanol decreased lipid production by 17.7%. However, when methanol was mixed with other impurities, its inhibition effect was alleviated due to the promoting effect of other impurities. Hence, crude glycerol could be used as a renewable and low-cost carbon source to replace pure glucose or glycerol for lipid preparation. (C) 2016 Elsevier Ltd. All rights reserved.
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