Journal
BIORESOURCE TECHNOLOGY
Volume 219, Issue -, Pages 114-122Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.07.100
Keywords
L-lactic acid; Bacillus coagulans; Lignocellulosic hydrolysates; Open continuous fermentation; Membrane cell-recycle
Funding
- Open Funds of the Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, China [2015009]
- Youth Science and Technology Foundation of Sichuan Province [2015JQO047]
- Special Fund for Agro-scientific Research in the Public Interest [201403019]
- Sichuan Key Technology RD Program [2014NZ0045]
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A thermophilic strain Bacillus coagulans (NBRC 12714) was employed to produce L-lactic acid from corn stover hydrolysate in membrane integrated continuous fermentation. The strain NBRC 12714 metabolized glucose and xylose by the Embden-Meyerhof-Parnas pathway (EMP) and the pentose phosphate pathway (PPP), producing L-lactic acid with optical purity >99.5%. The overall L-lactic acid titer of 92 g/l with a yield of 0.91 g/g and a productivity of 13.8 g/l/h were achieved at a dilution rate of 0.15 h(-1). The productivity obtained was 1.6-fold than that of conventional continuous fermentation without cell recycling, and also was the highest among the relevant studies ever reported. These results indicated that the process developed had great potential for economical industrial production of L-lactic acid from lignocellulosic biomass. (C) 2016 Elsevier Ltd. All rights reserved.
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