Journal
OCULAR IMMUNOLOGY AND INFLAMMATION
Volume 29, Issue 1, Pages 29-42Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/09273948.2019.1662060
Keywords
RelB; NF-?b; CCL2; conjunctiva; inflammation; surgery
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The study found that RelB was highly induced in postoperative conjunctival inflammation, potentially playing a specific anti-inflammatory role by selectively modulating CCL2 secretion.
Purpose: This study investigated the involvement of NF-kB in regulating postoperative conjunctival inflammation. Methods: Experimental surgery was performed as described for the mouse model of conjunctival scarring. Expression of NF-?B in postoperative conjunctival tissues or conjunctival fibroblasts were assessed by real-time PCR, immunoblotting and immunofluorescence analyses. Downregulation of RelB was achieved using small interfering RNA. Cellular cytokine secretion was determined using multiplex cytokine assay. Results: RelB was the most highly induced member of the NF-kB family on day 2 post-surgery. Elevated RelB may be found associated with vimentin-positive cells and fibroblasts in vivo and in vitro. In conjunctival fibroblasts, RelB may be induced by TNF-? but not TGF-?2 while its silencing caused selective induction of CCL2 secretion by both basal and TNF-?-stimulated fibroblasts. Conclusions: High RelB induction in the inflammatory phase and the selective modulation of CCL2 suggest a specific anti-inflammatory role for RelB in the postoperative conjunctiva.
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