Journal
BIORESOURCE TECHNOLOGY
Volume 211, Issue -, Pages 200-208Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.02.135
Keywords
Ammonia fibre expansion (AFEX); Oil palm empty fruit bunch (OPEFB) fibre; Biohydrogen; Oil palm residues; Biomass characterisation
Funding
- Universiti Kebangsaan Malaysia-Yayasan Sime Darby (UKM-YSD) Chair in Sustainable Development: Zero Waste Technology for the Palm Oil Industry
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Oil palm empty fruit bunch (OPEFB) fibre is widely available in Southeast Asian countries and found to have 60% (w/w) sugar components. OPEFB was pretreated using the ammonia fibre expansion (AFEX) method and characterised physically by the Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. The results show that there were significant structural changes in OPEFB after the pretreatment step, and the sugar yield after enzymatic hydrolysis using a cocktail of Cellic Ctec2 (R) and Cellic Htec2 (R) increased from 0.15 g g(-1) of OPEFB in the raw untreated OPEFB sample to 0.53 g g(-1) of OPEFB in AFEX-pretreated OPEFB (i.e. almost a fourfold increase in sugar conversion), which enhances the economic value of OPEFB. A biohydrogen fermentability test of this hydrolysate was carried out using a locally isolated bacterium, Enterobacter sp. KBH6958. The biohydrogen yield after 72 h of fermentation was 1.68 mol H-2 per mol sugar. Butyrate, ethanol, and acetate were the major metabolites. (C) 2016 Elsevier Ltd. All rights reserved.
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