Journal
BIORESOURCE TECHNOLOGY
Volume 219, Issue -, Pages 466-478Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.08.007
Keywords
Candida antarctica lipase A (CAL-A); n-3 polyunsaturated fatty acids (n-3 PUFAs); Monoacylglycerols (MAGs); Ethanolysis; Fish oil; Microalgae oil
Funding
- Key Projects of Department of Science and Technology of Fujian Province of China [2015N0007]
- Hu Guozan Study-Abroad for Graduates of Fujian Normal University, Department of Ocean and Fisheries of Fujian Province of China [[2015]06]
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Declining quantity/quality of available n-3 polyunsaturated fatty acids (n-3 PUFAs) resources demand innovative technology to concentrate n-3 PUFAs from low quality oils into value-added products/health-beneficial ingredients rich in n-3 PUFAs. This work proposed the catalytic property and specificity of an ideal enzyme required to tackle this task and identified Candida antarctica lipase A (CAL-A) is such a near-ideal enzyme in practice, which concentrates n-3 PUFAs from 25% to 27% in oils to a theoretically closer value 90% in monoacylglycerols (MAGs) via one-step enzymatic ethanolysis. Non-regiospecificity and high non-n-3 PUFAs preference of CAL-A are the catalytic feature to selectively cleave non-n-3 PUFAs in all 3 positions of triacylglycerols (TAGs); while high ethanol/TAGs ratio, low operation temperature and high tolerance to polar ethanol are essential conditions beyond biocatalyst itself. C-13 Nuclear magnetic resonance (C-13 NMR) analysis and competitive factor estimation verified the hypothesis and confirmed the plausible suggestion of catalytic mechanism of CAL-A. (C) 2016 Elsevier Ltd. All rights reserved.
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