Journal
NUCLEIC ACIDS RESEARCH
Volume 47, Issue 21, Pages 11007-11019Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz865
Keywords
-
Categories
Funding
- Welch Foundation [F-1808, F-1654]
- National Institutes of Health [GM124141]
- Arnold O. Beckman Postdoctoral Fellowship [GM124141]
Ask authors/readers for more resources
Precision genome editing technologies have transformed modern biology. These technologies have arisen from the redirection of natural biological machinery, such as bacteriophage lambda proteins for recombineering and CRISPR nucleases for eliciting site-specific double-strand breaks. Less well-known is a widely distributed class of bacterial retroelements, retrons, that employ specialized reverse transcriptases to produce noncoding intracellular DNAs. Retrons' natural function and mechanism of genetic transmission have remained enigmatic. However, recent studies have harnessed their ability to produce DNA in situ for genome editing and evolution. This review describes retron biology and function in both natural and synthetic contexts. We also highlight areas that require further study to advance retron-based precision genome editing platforms.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available