Journal
BIORESOURCE TECHNOLOGY
Volume 219, Issue -, Pages 710-715Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.08.035
Keywords
Synergistic effect; Xylanase; Cellulose; Corncob; Corn stover; Rice straw
Funding
- National Natural Science Foundation of China
- Science and Technology Support Program of Hubei Province [2015BBA177]
- National High Technology Research and Development Program (863 Program) [2012AA020403, 2012AA02A701]
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Synergistic combination of cellulase and xylanase has been performed on pre-treated substrates in many previous studies, while few on natural substrates. In this study, three unpretreated lignocellulosic substrates were studied, including corncob, corn stover, and rice straw. The results indicated that when the mixed cellulase and xylanase were applied, reducing sugar concentrations were calculated as 19.53, 15.56, and 17.35 mg/ml, respectively, based on the 3,5 dinitrosalicylic acid (DNS) method. Compared to the treatment with only cellulose, the hydrolysis yields caused by mixed cellulase and xylanase were improved by 133%, 164%, and 545%, respectively. In addition, the conversion yield of corncob, corn stover, and rice straw by cellulase-xylanase co-treatment reached 43.9%, 48.5%, and 40.2%, respectively, based on HPLC analysis, which confirmed the synergistic effect of cellulase-xylanase that was much higher than either of the single enzyme treatment. The substrate morphology was also evaluated to explore the synergistic mechanism of cellulase-xylanase. (C) 2016 Elsevier Ltd. All rights reserved.
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