Localization of fungal endophytes through histological study in healthy tissues of Murraya koenigii (L.) Spreng

Although endophytic fungi are widespread in almost all plants in the environment, few is known about their distribution in relation to specific plant cells and tissues. The aim was to locate endophytic fungi inside healthy leaflets of Murraya koenigii. The leaflet sections after staining with lactophenol cotton blue exhibited the presence of endophytic fungi in intercellular spaces of vascular bundles. In transverse sections at 1000x magnification, scattered endophytic hyphae were found between vessels of xylem tissues.