A biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse

In this study, a co-production of two high value-added products, glucose and xylooligosaccharides (XOS), was investigated by utilizing sugarcane bagasse (SB) within a multi-product bio-refinery framework optimized by Box-Behnken design-based response surface methodology. The developed process resulted in a maximum cellulose conversion of xylan-removed SB, 98.69 +/- 1.30%, and a maximum extracted SB xylan conversion into XOS (xylobiose and xylotriose) of 57.36 +/- 0.79% that was the highest SB xylan conversion reported in the literature, employing cellulase from Penicillium oxalicum EU2106 and recombinant endo-beta-1,4-xylanase in Pichia pastoris. Consequently, a mass balance analysis showed that the maximum yields of glucose and XOS were 34.43 +/- 0.32 g and 5.96 +/- 0.09 g per 100 g raw SB. Overall, this described process may be a preferred option for the comprehensive utilization of SB. (C) 2016 Elsevier Ltd. All rights reserved.