Journal
BIORESOURCE TECHNOLOGY
Volume 209, Issue -, Pages 297-304Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.03.037
Keywords
Inhibition; Lactic acid; Bacteria; Lignocellulosic by-products; Screening
Funding
- BE-Basic
- DSM
- Corbion
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Sugars obtained from pretreated lignocellulose are interesting as substrate for the production of lactic acid in fermentation processes. However, by-products formed during pretreatment of lignocellulose can inhibit microbial growth. In this study, a small-scale rapid screening method was used to identify inhibitory effects of single and combined by-products on growth of lactic acid producing microorganisms. The small-scale screening was performed in 48-well plates using 5 bacterial species and 12 by-products. Large differences were observed in inhibitory effects of by-products between different species. Predictions can be made for growth behaviour of different micro-organisms on acid pretreated or alkaline pretreated bagasse substrates using data from the small-scale screening. Both individual and combined inhibition effects were shown to be important parameters to predict growth. Synergy between coumaric acid, formic acid and acetic acid is a key inhibitory parameter in alkaline pretreated lignocellulose, while furfural is a key inhibitor in acid pretreated lignocellulose. (C) 2016 Elsevier Ltd. All rights reserved.
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