Journal
BIORESOURCE TECHNOLOGY
Volume 204, Issue -, Pages 98-105Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.12.070
Keywords
Wet and dry processing; Miscanthus x giganteus (MxG); Dilute sulfuric acid pretreatment; Simultaneous saccharification and fermentation; Bioethanol
Funding
- USDA-CSREES-Evans-Allen Project [NCX-272-5-13-130-1]
- Direct For Education and Human Resources
- Division Of Human Resource Development [1242152] Funding Source: National Science Foundation
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The effects of wet and dry processing of miscanthus on bioethanol production using simultaneous saccharification and fermentation (SSF) process were investigated, with wet samples showing higher ethanol yields than dry samples. Miscanthus grown with no fertilizer, with fertilizer and with swine manure were sampled for analysis. Wet-fractionation was used to separate miscanthus into solid and liquid fractions. Dilute sulfuric acid pretreatment was employed and the SSF process was performed with saccharomyces cerevisiae and a cocktail of enzymes at 35 degrees C. After pretreatment, cellulose compositions of biomass of the wet samples increased from 61.0-67.0% to 77.0-87.0%, which were higher than the compositions of dry samples. The highest theoretical ethanol yield of 88.0% was realized for wet processed pretreated miscanthus, grown with swine manure. Changes to the morphology and chemical composition of the biomass samples after pretreatment, such as crystallinity reduction, were observed using SEM and FTIR. These changes improved ethanol production. (C) 2016 Elsevier Ltd. All rights reserved.
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