Journal
BIORESOURCE TECHNOLOGY
Volume 205, Issue -, Pages 82-89Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.01.034
Keywords
ABE fermentation; Corn stalk juice; Corn stalk bagasse; Cell immobilization
Funding
- National Basic Research Program of China [2013CB733600, 2012CB725200]
- National Nature Science Foundation of China [21390202, 21276017, 21476015]
- National high-Tech R&D Program of China [2012AA021404, 2014AA021903]
- National Key Scientific Instruments and Equipment Development Special Fund [2012YQ0401400302]
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In order to make full use of the fresh corn stalk, the sugar containing juice was used as the sole substrate for acetone-butanol-ethanol production without any nutrients supplement, and the bagasse after squeezing the juice was used as the immobilized carrier. A total 21.34 g/L of ABE was produced in batch cells immobilization system with ABE yield of 0.35 g/g. A continuous fermentation containing three stages with immobilized cells was conducted and the effect of dilution rate on fermentation was investigated. As a result, the productivity and ABE solvents concentration reached 0.80 g/L h and 19.93 g/L, respectively, when the dilution rate in each stage was 0.12/h ( corresponding to a dilution rate of 0.04/h in the whole system). And the long-term operation indicated the continuous multiple stages ABE fermentation process had good stability and showed the great potential in future industrial applications. (C) 2016 Elsevier Ltd. All rights reserved.
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