Journal
BIORESOURCE TECHNOLOGY
Volume 219, Issue -, Pages 430-438Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.07.138
Keywords
Clostridium; Butanol; Xylan; No acetone/ethanol formation; Carbon catabolite derepression
Funding
- National Research Foundation, Prime Minister's Office, Singapore [NRF-CRP5-2009-05]
- National Natural Science Foundation of China [50978246]
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Production of lignocellulosic butanol has drawn increasing attention. However, currently few microorganisms can produce biofuels, particularly butanol, from lignocellulosic biomass via simultaneous saccharification and fermentation. Here we report discovery of a wild-type, mesophilic Clostridium sp. strain MF28 that ferments xylan to produce butanol (up to 3.2 g/L) without the addition of saccharolytic enzymes and without any chemical pretreatments. Application of selective pressure from 2-deoxy-Dglucose facilitated isolation of strain MF28, which exhibits inactivation of genes (gid and ccp genes) responsible for carbon catabolite repression, thus allowing strain MF28 to simultaneously ferment a combination of glucose (30 g/L), xylose (15 g/L), and arabinose (15 g/L) to produce 11.9 g/L of butanol. Strain MF28 possesses several unique features: (i) non-sporulating, (ii) no acetone/ethanol, (iii) complete hemicellulose-binding enzymatic domain, and (iv) absence of carbon catabolite repression. These unique characteristics demonstrate the industrial potential of strain MF28 for cost-effective biofuel generation from lignocellulosic biomass. (C) 2016 Elsevier Ltd. All rights reserved.
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