Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 39, Issue 4, Pages 685-693Publisher
SPRINGER
DOI: 10.1007/s00449-016-1549-1
Keywords
Stachybotrys microspora; Halophile; Neutral endoglucanase; Organic solvents; Barley-beta-glucan
Funding
- Ministry of Higher Education and Scientific Research, Tunisia
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The production of cellulases from Stachybotrys microspora strain (A19) has been improved by fed-batch fermentation on Avicel cellulose 10 mg/ml. An endoglucanase EG2 was purified to homogeneity. This cellulase has a molecular mass estimated to 50 kDa when analyzed by a denaturant gel electrophoresis. It exhibited an optimal activity at 50 A degrees C, pH 7.0 and 0.85 M NaCl. Specifically, these results show the thermo-active, alkali-tolerant and halo-tolerant properties of EG2. In addition, this endoglucanase showed its highest activity on barley-beta-glucan, compared to the CMC. Moreover, it was less active on Avicel cellulose. Furthermore, the EG2 activity was stimulated in the presence of EDTA, urea and beta-mercaptoethanol whereas it was reduced in the presence of SDS. This cellulase was highly stable in the presence of organic solvents such as acetone and n-hexane. TLC showed that the main hydrolysis products from EG2 were cellobiose and glucose. This fungal endoglucanase could be potentially important in the conversion of grass-derived biomass into fermentable sugars.
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