Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 39, Issue 11, Pages 1785-1791Publisher
SPRINGER
DOI: 10.1007/s00449-016-1654-1
Keywords
Edible oil; Releasing intracellular product; Whole cell biocatalyst; Cell suspension culture; Monascus pigments
Funding
- National Natural Science Foundation of China [21276155]
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Selective releasing intracellular product in Triton X-100 micelle aqueous solution to prepare whole cell biocatalyst is a novel strategy for biosynthesis of Monascus pigments, in which cell suspension culture exhibits some advantages comparing with the corresponding growing cell submerged culture. In the present work, the nonionic surfactant Triton X-100 was successfully replaced by edible plant oils for releasing intracellular Monascus pigments. High concentration of Monascus pigments (with absorbance nearly 710 AU at 470 nm in the oil phase, normalized to the aqueous phase volume approximately 142 AU) was achieved by cell suspension culture in peanut oil-water two-phase system. Furthermore, the utilization of edible oil as extractant also fulfills the demand for application of Monascus pigments as natural food colorant.
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