Journal
NATURE METHODS
Volume 16, Issue 11, Pages 1101-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-019-0584-7
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Funding
- German Research Foundation through the Emmy Noether Program [DFG JU 2957/1-1, SFB1032, A11]
- European Research Council through an ERC Starting Grant (MolMap) [680241]
- Allen Distinguished Investigator Program through the Paul G. Allen Frontiers Group
- Max Planck Foundation
- Max Planck Society
- QBM graduate school
- International Max Planck Research School for Molecular and Cellular Life Sciences
- European Research Council (ERC) [680241] Funding Source: European Research Council (ERC)
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DNA points accumulation in nanoscale topography (DNA-PAINT) is a relatively easy-to-implement super-resolution technique. However, image acquisition is slow compared to most other approaches. Here, we overcome this limitation by designing optimized DNA sequences and buffer conditions. We demonstrate our approach in vitro with DNA origami and in situ using cell samples, and achieve an order of magnitude faster imaging speeds without compromising image quality or spatial resolution. This improvement now makes DNA-PAINT applicable to high-throughput studies.
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