Journal
NATURE
Volume 574, Issue 7778, Pages 365-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41586-019-1652-y
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Funding
- Wellcome Human Cell Atlas Strategic Science Support [WT211276/Z/18/Z]
- Wellcome [WT107931/Z/15/Z, WT206194, WT110104/Z/15/Z]
- Lister Institute for Preventive Medicine
- NIHR
- Newcastle Biomedical Research Centre
- ERC Consolidator award
- EU MRG-Grammar award
- St. Baldrick's Foundation
- Royal Society Fellowship
- European Haematology Association
- Wellcome
- MRC
- Manton Foundation
- Klarman Cell Observatory
- BBSRC [BB/P002293/1] Funding Source: UKRI
- MRC [MC_PC_16040, MR/R006237/1] Funding Source: UKRI
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Definitive haematopoiesis in the fetal liver supports self-renewal and differentiation of haematopoietic stem cells and multipotent progenitors (HSC/MPPs) but remains poorly defined in humans. Here, using single-cell transcriptome profiling of approximately 140,000 liver and 74,000 skin, kidney and yolk sac cells, we identify the repertoire of human blood and immune cells during development. We infer differentiation trajectories from HSC/MPPs and evaluate the influence of the tissue microenvironment on blood and immune cell development. We reveal physiological erythropoiesis in fetal skin and the presence of mast cells, natural killer and innate lymphoid cell precursors in the yolk sac. We demonstrate a shift in the haemopoietic composition of fetal liver during gestation away from being predominantly erythroid, accompanied by a parallel change in differentiation potential of HSC/MPPs, which we functionally validate. Our integrated map of fetal liver haematopoiesis provides a blueprint for the study of paediatric blood and immune disorders, and a reference for harnessing the therapeutic potential of HSC/MPPs.
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