A new indole alkaloid with HUVEC proliferation activities from Nauclea officinalis

A new indole alkaloid, namely naucleofficine H (1), was obtained from the aqueous extract of Nauclea officinalis, together with four known alkaloids, vincosamide (2), strictosamide (3), angustoline (4) and pumiloside (5). Their structures were characterized by analyzing their physicochemical data including NMR, and HRMS. In addition, five compounds were tested for their proliferation activities. The expression of vascular endothelial growth factor (VEGF), extra-cellular signal-regulated protein kinase 1 and 2 (ERK) and phosphorylation of ERK 1/2 (p-ERK) were also detected in HUVEC treated withbioactive compounds using western blotting. The result showed that these compounds could promote HUVEC cell proliferation. Compounds 3 and 5 could up-regulate VEGF and p-ERK in HUVEC.

Automated summary

A new indole alkaloid, naucleofficine H, was isolated from Nauclea officinalis, along with four known alkaloids. These compounds were found to promote HUVEC cell proliferation, with compounds 3 and 5 up-regulating the expression of VEGF and p-ERK in HUVEC cells.