Journal
MOVEMENT DISORDERS
Volume 35, Issue 2, Pages 268-278Publisher
WILEY
DOI: 10.1002/mds.27907
Keywords
formalin-fixed paraffin-embedded (FFPE); Parkinson's disease (PD); pathological alpha-synuclein (paS); real-time quaking-induced conversion (RT-QuIC); submandibular gland (SMG)
Categories
Funding
- National Institutes of Health [ES026892, NS100090, NS088206, NS112008]
- U.S. Army Medical Research Materiel Command [W81XWH-17-PRP-IIRA, W81XWH1810106]
- U.S. Department of Defense (DOD) [W81XWH1810106] Funding Source: U.S. Department of Defense (DOD)
Ask authors/readers for more resources
Background Identification of a peripheral biomarker is a major roadblock in the diagnosis of PD. Immunohistological identification of p-serine 129 alpha-synuclein in the submandibular gland tissues of PD patients has been recently reported. Objective We report on a proof-of-principle study for using an ultra-sensitive and specific, real-time quaking-induced conversion assay to detect pathological alpha-synuclein in the submandibular gland tissues of PD patients. Methods The alpha-synuclein real-time quaking-induced conversion assay was used to detect and quantify pathological alpha-synuclein levels in PD, incidental Lewy body disease, and control submandibular gland tissues as well as in formalin-fixed paraffin-embedded sections. Results We determined the quantitative seeding kinetics of pathological alpha-synuclein present in submandibular gland tissues from autopsied subjects using the alpha-synuclein real-time quaking-induced conversion assay. A total of 32 cases comprising 13 PD, 3 incidental Lewy body disease, and 16 controls showed 100% sensitivity and 94% specificity. Interestingly, both PD and incidental Lewy body disease tissues showed 100% concordance for elevated levels of pathological alpha-synuclein seeding activity compared to control tissues. End-point dilution kinetic analyses revealed that the submandibular gland had a wide dynamic range of pathological alpha-synuclein seeding activity. Conclusions Our results are the first to demonstrate the utility of using the real-time quaking-induced conversion assay on peripherally accessible submandibular gland tissues and formalin-fixed paraffin-embedded tissue sections to detect PD-related pathological changes with high sensitivity and specificity. Additionally, the detection of seeding activity from incidental Lewy body disease cases containing immunohistochemically undetected pathological alpha-synuclein demonstrates the alpha-synuclein real-time quaking-induced conversion assay's potential utility for identifying prodromal PD in submandibular gland tissues. (c) 2019 International Parkinson and Movement Disorder Society
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available