Journal
MOLECULES
Volume 24, Issue 20, Pages -Publisher
MDPI
DOI: 10.3390/molecules24203763
Keywords
grape canes; polyphenols; LC-LTQ-Orbitrap; Vitis vinifera; by-products
Funding
- CONICYT [PCI REDES170051, PFCHA/DOCTORADO BECAS CHILE/2017-72180476]
- CICYT from the Spanish Ministry of Industry, Economy and Competitivity [AGL2016-79113-R]
- Centro de Investigacion Biomedica en Red, (CIBEROBN)
- Generalitat de Catalunya [2017SGR196]
- CONICYT PIA/APOYO CCTE [AFB170007]
- Ministry of Science, Innovation and Universities [RYC-2016-19355, BES-2017-080017]
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Grape canes (Vitis vinifera L.) are a viticulture industry by-product with an important content of secondary metabolites, mainly polyphenols with a broad spectrum of demonstrated health benefits. Grape canes, therefore, have considerable economic potential as a source of high-value phytochemicals. In this work, liquid chromatography coupled with electrospray ionization hybrid linear trap quadrupole-Orbitrap mass spectrometry (LC-LTQ-Orbitrap) was used for the comprehensive identification of polyphenolic compounds in grape canes. Identification of polyphenols was performed by comparing their retention times, accurate mass measured, and mass fragmentation patterns with those of reference substances or available data in the literature. A total of 75 compounds were identified, including phenolic acids, flavanols, flavonols, flavanonols, flavanones, and stilbenoids. The most abundant polyphenols were proanthocyanidins and stilbenoids and their oligomers. Moreover, the high-resolution mass spectrometry analysis revealed the occurrence of 17 polyphenols never described before in grape canes, thereby providing a more complete polyphenolic profile of this potentially valuable by-product.
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