Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 30, Issue 22, Pages 2737-2740Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E18-11-0694
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Funding
- MRC Next Generation imaging grant [MR/K015613/1]
- MRC [MR/K015613/1] Funding Source: UKRI
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Antibodies have long been the main approach used for localizing proteins of interest by light microscopy. In the past 5 yr or so, and with the advent of superresolution microscopy, the diversity of tools for imaging has rapidly expanded. One main area of expansion has been in the area of nanobodies, small single-chain antibodies from camelids or sharks. The other has been the use of artificial scaffold proteins, including Affimers. The small size of nanobodies and Affimers compared with the traditional antibody provides several advantages for superresolution imaging.
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