Journal
BIOPHYSICAL JOURNAL
Volume 111, Issue 5, Pages 999-1007Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2016.07.035
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Funding
- Integrated Lipidology Program of RIKEN
- Japan Society for the Promotion of Science (JSPS) [25293015, 26620141]
- Center of Innovation Program of the Japan Science and Technology Agency (JST)
- Naito Foundation
- University of Nagoya through the HPCI System Research Project [hp140105]
- Grants-in-Aid for Scientific Research [26620141, 25293015] Funding Source: KAKEN
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Sphingomyelin (SM) is a major sphingolipid in mammalian cells that forms specific lipid domains in combination with cholesterol (Chol). Using molecular-dynamics simulation and density functional theory calculation, we identified a characteristic Raman band of SM at similar to 1643 cm(-1) as amide I of the SM cluster. Experimental results indicate that this band is sensitive to the hydration of SM and the presence of Chol. We showed that this amide I Raman band can be utilized to examine the membrane distribution of SM. Similarly to SM, ceramide phosphoethanolamine (CerPE) exhibited an amide I Raman band in almost the same region, although CerPE lacks three methyl groups in the phosphocholine moiety of SM. In contrast to SM, the amide I band of CerPE was not affected by Chol, suggesting the importance of the methyl groups of SM in the SM-Chol interaction.
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