Journal
MICROCHIMICA ACTA
Volume 186, Issue 12, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3887-6
Keywords
Metal doping; Enzyme mimetic; Nanozyme; Tetramethylbenzidine; Colorimetry; Ascorbic acid assay; Oxidase-like activity; Optical sensor; Inhibitory effect; Blood serum
Categories
Funding
- Anhui Laboratory of Molecule-Based Materials Open Fund [fzj19006]
- National Natural Science Foundation of China [21303003, 21375003, 21705003]
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A colorimetric assay is presented for the determination of ascorbic acid (AA). Manganese(II) doped carbon dots (Mn-CDs) were prepared by a convenient hydrothermal route and are shown to possess oxidase-like catalytic ability. They catalyze the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) by dissolved oxygen to form a blue colored product (oxTMB). AA can reduce blue oxTMB to colorless TMB. The fading of the blue color (measured at 652 nm) can be applied for quantifying AA in the 50 to 2500 nM concentration range and with a 9 nM detection limit. The method was successfully used for the quantitation of AA in real samples.
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