Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 104, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2019.109955
Keywords
Iron oxide nanoparticles; Calcium phosphate scaffold; Dental stem cells; Osteoinduction; Bone engineering
Categories
Funding
- National Science Foundation of China [81771044]
- National Key Research [2016YFA0201704/2016YFA0201700, 2017YFA0104301]
- Jiangsu Provincial Medical Youth Talent [QNRC2016853]
- Qing Lan Project, Southeast University-Nanjing Medical University Cooperative Research Project [2242018K3DN16]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [2018-87]
- Collaborative Innovation Center of Suzhou Nano Science and Technology, University of Maryland Baltimore
- University of Maryland School of Dentistry bridge fund
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Calcium phosphate cement (CPC), functionalized with iron oxide nanoparticles (IONP), is of great promise to promote osteoinduction and new bone formation. In this work, the IONP powder was added into the CPC powder to fabricate CPC + IONP scaffolds and the effects of the novel composite on bone matrix formation and osteogenesis of human dental pulp stem cells (hDPSCs) were explored. A series of CPC + IONP magnetic scaffolds with different IONP contents (1%, 3% and 6%) were fabricated using 5% chitosan solution as the cement liquid. Western blotting and RT-PCR were used to analyze the signaling pathway. The IONP incorporation substantially enhanced the performance of CPC + IONP, with increases in both mechanical strength and cellular activities. The IONP addition greatly promoted the osteogenesis of hDPSCs, elevating the ALP activity, the expression of osteogenic marker genes and bone matrix formation with 1.5-2-fold increases. The 3% IONP incorporation showed the most enhancement among all groups. Activation of the extracellular signal-related kinases WNT/beta-catenin in DPSCs was observed, and this activation was attenuated by the WNT inhibitor DKK1. The results indicated that the osteogenic behavior of hDPSCs was likely driven by CPC + IONP via the WNT signaling pathway. In conclusion, incorporate IONP into CPC scaffold remarkably enhanced the spreading, osteogenic differentiation and bone mineral synthesis of stem cell. Therefore, this method had great potential for bone tissue engineering. The novel CPC + IONP composite scaffolds with stem cells are promising to provide an innovative strategy to enhance bone regenerative therapies.
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