Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 103, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2019.109763
Keywords
M. koenigii berry; Selenium nanoparticles; Aedes aegypti; Antibiofilm; In vitro; Artemia nauplii
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Funding
- AURF-Research fellowship from Alagappa University, Karaikudi, India [Ph.D./1620/AURF Fellowship/2017]
- RUSA Phase 2.0 grant, Policy (TNMulti-Gen), Dept. of Edn. Govt of India [F.24-51/2014-U]
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In the present study, we investigated the larvicidal and bacteriostatic activity of biosynthesized selenium nanoparticles using aqueous berry extract of Murraya koenigii (Mk-Se NPs). The synthesized Mk-Se NPs were characterized using UV-visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis. XRD analysis revealed the crystalline nature of Mk-Se NPs as hexagonal. The FTIR spectra of Mk-Se NPs exhibited a strong peak at 3441 cm(-1) corresponding to the O-H group. SEM and TEM analysis showed that the Mk-Se NPs were spherical in shape with a size between 50 and 150 nm. EDX peaks confirm the presence of 73.38% of selenium and 26.62% of oxide in Mk-Se NPs. Mk-Se NPs showed significant larvicidal property against the 4th instar larvae of a dengue fever-causing vector Aedes aegypti with LC50- - 3.54 mu g mL(-1) and LC90- - 8.128 mu g mL(-1) values. Mk-Se NPs displayed anti-bacterial activity against Gram-positive (Enterococcus faecalis & Streptococcus mutans) and Gram-negative (Shigella sonnei & Pseudomonas aeruginosa) bacteria at 40 and 50 mu g mL(-1). In addition, Mk-Se NPs reduced bacterial biofilm thickness extensively at 25 mu g mL(-1). The high antioxidant property at 50 mu g mL(-1) and low hemolysis activity till 100 mu g mL(-1) proved the biocompatible nature of Mk-Se NPs. In vitro and in vivo toxicity assessment of Mk-Se NPs showed low cytotoxicity against RAW 264.7 macrophages and Artemia nauplii. Together, our results suggest the potential application of Mk-Se NPs as a nano-biomedicine.
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