Journal
MARINE DRUGS
Volume 17, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/md17110597
Keywords
cucumarioside A(2)-2; doxorubicin; ehrlich ascites carcinoma; MDR; sea cucumbers; triterpene glycosides
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Funding
- FEB RAS [19-MHT-017]
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The effect of treatment of Ehrlich ascites carcinoma (EAC) cells with multidrug resistance by holothurian triterpene glycoside, cucumarioside A(2)-2 (CA(2)-2) was evaluated. Calcein-AM efflux assay and doxorubicin (DOX) uptake and retention measurement in cancer cells, as well as determination of DOX cytotoxic and anticancer effects were applied. Treatment of EAC cells with CA(2)-2 (0.01-0.1 mu M) blocked Calcein-AM and DOX efflux from cancer cells and increased the accumulation and cytotoxicity of DOX in EAC cells. Moreover, pre-treatment of mice with EAC by CA(2)-2 (10 mu g/kg/5 days, intraperitoneal injection (i.p.)), then transplantation of tumor cells into fresh animals and subsequent treatment of these mice with DOX (2 mg/kg/3 days i.p.) significantly increased average life span (ALS) of mice bearing a tumor and therefore boosted the antitumor effect of doxorubicin in vivo.
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