Journal
LUMINESCENCE
Volume 35, Issue 2, Pages 222-230Publisher
WILEY
DOI: 10.1002/bio.3717
Keywords
energy transfer; fluorescent nanomaterials; carbon dots; beta-glucosidase; cell imaging
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Funding
- Fundamental Research Funds for the Central Universities [XDJK2019C055]
- National Natural Science Foundation of China [21535006, 21705130]
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Energy transfer engineering based on fluorescent probes for directly sensing enzyme activities are in great demand as enzyme-mediated transformations, which are central to all biological processes. Here, a fluorescence carbon dot (CD)-based assay exhibiting selective responses to the quantitation of beta-glucosidase and the effect of its inhibitor was developed. The most common substrate, para-nitrophenyl-beta-d-glucopyranoside (pNPG) was hydrolyzed by beta-glucosidase to release p-nitrophenol (pNP), which can efficiently quench fluorescence of CDs via an inner filter effect and electron transfer. However, in the presence of inhibitors of beta-glucosidase, the fluorescence intensity gradually recovered as the concentration of inhibitors increased. Therefore, the enzyme-triggered fluorescence turn-off/turn-on of specific CDs successfully achieved sensitive detection of beta-glucosidase and monitored the effect of its inhibitors. This new strategy was applied to detect beta-glucosidase and monitor beta-glucosidase inhibitor in hepatoma cells using cell imaging. All results suggest that the new method is sensitive and promising for use in cancer diagnosis and treatment.
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