Journal
LANGMUIR
Volume 35, Issue 50, Pages 16528-16535Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.9b02488
Keywords
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Funding
- Consejo Nacional de Ciencia y Tecnologia CONACYT, under project Fronteras de la Ciencia [2-1132]
- CONACYT
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We took advantage of the microflow hydrodynamics in the evaporation of sessile droplets to increase the height uniformity of thin lipid films for the subsequent electroformation of defect-free giant unilamellar vesicles (GUV). By serially casting progressively larger liposome suspension droplets on the same spot of an indium-tin-oxide (ITO) electrode, we managed to leverage the coffee ring effect (CRE) in the evaporation of each droplet to generate a smeared multilayer film of uniform thickness. This multidroplet technique of lipid film formation outperformed the traditional single-droplet deposition, improving the final quality of electroformed GUV samples. The proposed film formation technique constitutes a solvent-free method that results in a dramatic reduction (similar to 20x) in the appearance of undesirable structures like nonspherical (NSV), multilamellar (MLV), and multivesicular (MVV) vesicles.
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