Journal
JOURNAL OF SEPARATION SCIENCE
Volume 43, Issue 2, Pages 438-451Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201900641
Keywords
citrus reticulata semen; tandem mass spectrometry; traditional Chinese medicine; ultra high performance liquid chromatography
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Funding
- National Key R&D Program of China [2017YFC1701103]
- National Modern Agricultural Industrial Park of China [njf [2017] 110]
- National Natural Science Foundation of China [31401613]
- Cultivation Plan for High-Level University Academic Backbone of Guangzhou Medical University in 2017 [gydf [2017] 210]
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Citrus reticulata semen, a traditional Chinese medicinal material, has desirable medicinal and dietary properties. In this study, a method combining ultra high performance liquid chromatography with Q Exactive Orbitrap tandem mass spectrometry was established and validated for the identification and analysis of the chemical components of C. reticulata semen for the first time. The evaluation of different retention times and fragmentation characteristics, as well as comparative analysis with the literature, resulted in the identification of 35 chemical constituents, including 21 flavonoids and 14 other compounds. The 21 flavonoids derived from C. reticulata semen were reported for the first time. Seven of the chemical components of C. reticulata semen were quantitatively analyzed using the developed method under the optimal conditions. The results showed that the content of limonin, hesperidin, nobiletin, synephrine, tangeretin, 3,5,6,7,8,3 ',4 '-heptamethoxyflavone and 5-hydroxide-6,7,8,3 ',4 '-pentamethoxyflavone in C. reticulata semen was 11.1666, 0.0404, 0.0092, 0.0255, 0.0087, 0.0010, and 0.0008 mg/g, respectively. This study demonstrated that the ultra high performance liquid chromatography Q Exactive Orbitrap mass spectrometry based method can be used to rapidly and reliably analyze the chemical constituents of C. reticulata semen. These results provide a scientific basis for further studies of C. reticulata semen.
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