4.1 Article

High-throughput sequencing uncover Ficus tikoua Bur. chloroplast genome

Journal

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 29, Issue 2, Pages 171-182

Publisher

SPRINGER INDIA
DOI: 10.1007/s13562-019-00537-9

Keywords

Ficus tikoua Bur; Chloroplast genome; Genome annotation; Repetitive sequences

Funding

  1. National Natural Science Foundation of China [30971794]
  2. Science and Technology Foundation of Guizhou Province, China [QKHJC[2017]1004]
  3. Training Program for High-level Innovative Talents of Guizhou Province [QKHRC[2016]4020]
  4. Program for First-class Discipline Construction in Guizhou Province [QJKYF[2017]85]
  5. National Innovation Training Program for Undergraduate [2018109760007, 2018109 760008]
  6. Innovative Training Program for Undergraduate in Guizhou Province [2018520777, 2018520778, 2018520831, 20195200341, 20195201373, 2018520861]
  7. educational incubation program of Guiyang university [GYU-KJT[2019]-06, GYU-KJT[2019]-07]
  8. Foundation of Guiyang science and technology bureau [GYU-KYZ[2019-2020]PT13-02]
  9. Project for Major Research by Innovation Group in Guizhou Education Department [QJHKY[2018]027]

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Ficus tikoua Bur., called di guo, is a very important economic plant. However, we know little on its molecular mechanisms for a long time. In this study, three F. tikoua chloroplast genomes were first obtained through Illumina sequencing. The F. tikoua Bur. cp genomes exhibited a typical circular structure, including a pair of inverted repeats, a small single copy sequence, and a long single copy sequence. More than 100 genes and about 70 ncRNAs (rRNAs, tRNAs, snRNA, snoRNA, etc.) were identified in each cp genomes. Some repeats, including DNA element, long terminal repeat, small RNA, simple repeat and low complexity, were examined in the three complete cp genomes. In addition, phylogeny tree from six Ficus. species and five Morus. species in family Moracea were constructed for clarifying taxonomy. This data can help further the understanding of evolution and phylogenetic relationships in F. tikoua Bur.

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