Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 200, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2019.111654
Keywords
Photodynamic therapy; Microbicide; Phenothiazine; Enterococcus spp
Categories
Funding
- Brazilian National Council for Scientific and Technological Development - CNPq
- Bahia State Research Support Foundation FAPESB
- Coordination of Superior Level Staff Improvement CAPES
Ask authors/readers for more resources
The Enterococcus faecalis is a microorganism that causes multiple forms of resistance to a wide range of drugs used clinically. aPDT is a technique in which a visible light activates photosensitizer (PS), resulting in generation of reactive oxygen species that kill bacteria unselectively via an oxidative burst. aPDT is an alternative to antibiotics with the advantage of not causing resistance. The search for an alternative treatment of infections caused by E. faecalis, without using antibiotics, is off great clinical importance. The aim of present investigation was to assess the efficacy of using 3.32 eta g/mL of 1,9-dimethylmethylene blue (DMMB) as photosensitizer associated with the use of either Laser (lambda 660 nm) or LED (lambda 632 +/- 2 nm) using different energy densities (6, 12 and 18 J/cm(2)) to kill E. faecalis in vitro. Under different experimental conditions, 14 study groups, in triplicate, were used to compare the efficacy of the aPDT carried out with either the laser or LED lights using different energy densities associated to DMMB. The most probable number method (MPN) was used for quantitative analysis. Photodynamic antimicrobial effectiveness was directly proportional to the energy density used, reaching at 18 J/cm(2), 99.999998% reduction of the counts of E. faecalis using both light sources. The results of this study showed that the use of 3.32 eta g/mL of DMMB associated with the use 18 J/cm(2) of LED light (lambda 632 +/- 2 nm) reduced > 7-log counts of planktonic culture of E. faecalis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available