Journal
JOURNAL OF PHARMACEUTICAL SCIENCES
Volume 109, Issue 3, Pages 1373-1379Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.xphs.2019.11.014
Keywords
anti-infective(s); pharmacokinetics; clinical pharmacokinetics; HPLC (high-performance/pressure liquid chromatography); pharmacodynamics; microdialysis
Funding
- Health Research Foundation of Central Denmark Region, Denmark
- Elisabeth og Karl Ejnar Nis-Hansens Mindelegat Foundation
- Familien Hede Nielsen Foundation
- Laege Sofus Carl Emil Friis og Hustru Olga Doris Friis' Legat Foundation
- Christian Larsen og dommer Ellen Larsens Legat Foundation
- Torben og Alice Frimodt Foundation
- Helga og Peter Kornings Foundation
- A. P. Moller Foundation
- Orthopaedic Research in Aarhus Foundation, Denmark
- Department of Orthopaedic Surgery, Horsens Regional Hospital
- Orthopaedic Research Unit, Aarhus University Hospital
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Microdialysis is a valuable pharmacokinetic tool for obtaining samples of drug concentrations from tissues of interest. When an absolute tissue concentration is needed, a calibration of the microdialysis catheter is required. The use of an internal standard offers a number of advantages compared to standard calibration methods. However, meticulous validation both in vitro and in vivo is needed, as this method requires an internal standard with physiochemical similarities to the analyte of interest with no interference. A series of in vitro and in vivo setups were conducted to determine the relative recovery by gain and by loss for cefuroxime, with and without a constant meropenem concentration. The cefuroxime and meropenem concentrations were determined using ultra-HPLC. The main finding was that cefuroxime and meropenem relative recovery behaved similarly both in vitro and in vivo, signifying that meropenem is a representative internal standard for cefuroxime. Furthermore, cefuroxime relative recovery in vitro was not affected by either the cefuroxime concentration or the presence of meropenem, and the in vivo meropenem relative recovery was constant over 6 h. (c) 2020 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.
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