Journal
JOURNAL OF NEUROSCIENCE
Volume 40, Issue 1, Pages 159-170Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.0806-19.2019
Keywords
cerebellar nuclei; cerebellum; electrophysiology in vivo; Purkinje cells; temporal coding
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Funding
- Centre National pour la Recherche Scientifique
- Universite de Strasbourg
- Agence Nationale pour la Recherche [ANR-15-CE37-0001-01 CeMod]
- Fondation pour la Recherche Medicale [DEQ20140329514]
- NeuroTime Erasmus Mundus Joint Doctorate Neuroscience PhD program - (European Commission)
- European research council-advanced (ERC-adv)
- Dutch research council (NWO-ALW), The Netherlands Organisation for Health Research and Development (ZonMw), Koninklijke Nederlandse Akademie van Wetenschappen (KNAW) [UMS 3415]
- Agence Nationale de la Recherche (ANR) [ANR-15-CE37-0001] Funding Source: Agence Nationale de la Recherche (ANR)
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The cerebellum drives motor coordination and sequencing of actions at the millisecond timescale through adaptive control of cerebellar nuclear output. Cerebellar nuclei integrate high-frequency information from both the cerebellar cortex and the two main excitatory inputs of the cerebellum: the mossy fibers and the climbing fiber collaterals. However, how nuclear cells process rate and timing of inputs carried by these inputs is still debated. Here, we investigate the influence of the cerebellar cortical output, the Purkinje cells, on identified cerebellar nuclei neurons in vivo in male mice. Using transgenic mice expressing Channelrhodopsin2 specifically in Purkinje cells and tetrode recordings in the medial nucleus, we identified two main groups of neurons based on the waveform of their action potentials. These two groups of neurons coincide with glutamatergic and GABAergic neurons identified by optotagging after Chrimson expression in VGLUT2-cre and GAD-cre mice, respectively. The glutamatergic-like neurons fire at high rate and respond to both rate and timing of Purkinje cell population inputs, whereas GABAergic-like neurons only respond to the mean population firing rate of Purkinje cells at high frequencies. Moreover, synchronous activation of Purkinje cells can entrain the glutamatergic-like, but not the GABAergic-like, cells over a wide range of frequencies. Our results suggest that the downstream effect of synchronous and rhythmic Purkinje cell discharges depends on the type of cerebellar nuclei neurons targeted.
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