Journal
JOURNAL OF MOLECULAR RECOGNITION
Volume 33, Issue 3, Pages -Publisher
WILEY
DOI: 10.1002/jmr.2822
Keywords
catalase; hepatocyte; maltol; spectroscopy; toxic effect
Categories
Funding
- Science and Technology Development Plan of Shandong Province [2014GSF117027]
- Research Fund for the Doctoral Program of Higher Education and Ministry of Education of China [20130131110016, 708058]
- Cultivation Fund of the Key Scientific and Technical Innovation Project
- NSFC [U1806216, 21777088, 51608304]
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Maltol is a flavor additive that is widely used in the daily diet of humans, and its biosafety attention is concomitantly increasing. Catalase (CAT) is an antioxidant enzyme to maintain homeostasis in the tissue's environment of human body and protect cells from oxidative damages. The adverse effects of maltol to CAT activity within mouse hepatocytes as well as the structural and functional changes of CAT on molecular level were investigated by multiple spectroscopy techniques, enzyme activity experiments, and molecular docking. Results suggested that when the maltol concentrations reached to 8 x 10(-5) mol L-1, the viability of hepatocytes decreased to 93%, and CAT activity was stimulated by maltol to 111% than the control group after exposure for 24 hours. Changes in CAT activity on molecular level were consistent with those on cellular level. The fluorescence quenching of CAT by maltol was static with the forming of maltol-CAT complex. Moreover, ultraviolet-visible (UV-visible) absorption, synchronous fluorescence, and circular dichroism (CD) spectra reflected that the presence of maltol caused conformational change of CAT and made the CAT molecule skeleton loose and increased alpha-helix of CAT. Maltol mainly bound with CAT through hydrogen bond, and binding site that is near the heme ring in the enzyme activity center did not interact with its main amino acid residues. This study explores the combination between maltol and CAT, providing references for evaluating health damages caused by maltol.
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