Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 165, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2019.105703
Keywords
Escherichia coli O157; Escherichia coli O26; Quantitative real-time PCR; Sheep
Categories
Funding
- Food Institutional Research Measure [15/F/629]
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A quantitative PCR method is described for the detection and quantification of E. coli 0157 and 026 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values to the initial concentration of pathogen in the sheep sample.
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