Journal
JOURNAL OF MEDICINAL FOOD
Volume 23, Issue 1, Pages 79-89Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/jmf.2019.4476
Keywords
inflammation; interferon regulatory factor 3; lipopolysaccharide; Radix Isatidis; sepsis
Funding
- National Natural Science Foundation of China [81603156, 81573571]
- Excellent Academic Leaders Program of Shanghai [16XD1403500]
- Young Eastern Scholar Program [QD2016038]
- Chenguang Program [16CG49]
- program of High Level University Innovation Team
- program of Shanghai E-Research Institute of Bioactive Constituents in Traditional Chinese Medicine
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Endotoxic shock exhibits a considerably high mortality risk. It is defined as a systemic inflammatory response syndrome caused by a microbial infection. Radix Isatidis has anti-inflammatory, antiviral, and antipyretic effects and is used worldwide. This study investigated the antiendotoxin sepsis effects of an aqueous R. Isatidis extract (RIE) and explored the possible pharmacological molecular mechanisms. Male C57BL/6J mice were intravenously injected with 15 mg/kg lipopolysaccharide (LPS) to induce endotoxic shock. The results demonstrated that the survival rate of mice pretreated with RIE increased, and LPS-induced liver and lung damage were reduced by inhibiting inflammation. For elucidating detailed molecular mechanisms, we focused on LPS-induced transcription factors: nuclear factor-kappa B (NF-kappa B) and interferon regulatory factor 3 (IRF3). Our results demonstrated that the protective effects of RIE were strongly dependent on IRF3-induced interferon-beta, not on NF-kappa B-induced tumor necrosis factor-alpha and interleukin-1 beta. In addition, RIE suppressed the phosphorylation of IRF3, not NF-kappa B. In conclusion, this study revealed the antiendotoxic properties of RIE on LPS-induced sepsis and provided mechanistic evidence for the beneficial effects of RIE.
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