Purification, characterization, and bioactivity of two new polysaccharide fractions from Thelephora ganbajun mushroom

Two new polysaccharide fractions (TZP1-1 and TZP2-1) were obtained from the fruiting bodies of Thelephora ganbajun using DEAE-52 cellulose and Superdex 200 columns chromatography. The physiochemical characterization and biological activities of TZP1-1 and TZP2-1 were investigated. The relative molecular weight of TZP1-1 and TZP2-1 were 2.07 x 10(6) and 4,886 Da, respectively. TZP1-1 included mannose, rhamnose, galactose, and xylose (4:1:83.9:7.5), while TZP2-1 included mannose, glucose, galactose, and xylose (5.4:1:79.0:8.1). The Congo red experiment results confirmed that TZP2-1 had triple helix conformation. Furthermore, both TZP1-1 and TZP2-1 showed a certain cytotoxicity on HeLa and SH-SY5Y cells, while they exhibited a stronger inhibitory effect on HeLa than SH-SY5Y. Besides, the cytotoxicity of TZP1-1 was better than that of TZP2-1. Moreover, both of them exhibited a moderate inhibitory effect on alpha-amylase and alpha-glucosidase. These findings could promote the application of polysaccharides from T. ganbajun. Practical applications Thelephora ganbajun is an edible fungus widely distributed in Southwestern China. T. ganbajun polysaccharides as important active ingredients have not been reported. In this current study, two polysaccharides fractions (TZP1-1 and TZP2-1) were characterized, and their cytotoxicities and antidiabetic effect were also assayed. These findings could promote polysaccharides from T. ganbajun to be better application.