Journal
BIOORGANIC & MEDICINAL CHEMISTRY
Volume 24, Issue 21, Pages 5134-5147Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2016.08.025
Keywords
Burkholderia pseudomallei; Legionella pneumophila; Macrophage infectivity potentiator protein; Synthesis; Docking analysis; Structure-activity-relationships
Funding
- Deutsche Forschungsgemeinschaft (Bonn, Germany) [SFB 630]
- North Atlantic Treaty Organization (NATO, Brussels, Belgium)
- [DSTLX-1000094053]
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The bacteria Burkholderia pseudomallei and Legionella pneumophila cause severe diseases like melioidosis and Legionnaire's disease with high mortality rates despite antibiotic treatment. Due to increasing antibiotic resistances against these and other Gram-negative bacteria, alternative therapeutical strategies are in urgent demand. As a virulence factor, the macrophage infectivity potentiator (Mip) protein constitutes an attractive target. The Mip proteins of B. pseudomallei and L. pneumophila exhibit peptidyl-prolyl cis/trans isomerase (PPIase) activity and belong to the PPIase superfamily. In previous studies, the pipecolic acid moiety proved to be a valuable scaffold for inhibiting this PPIase activity. Thus, a library of pipecolic acid derivatives was established guided by structural information and computational analyses of the binding site and possible binding modes. Stability and toxicity considerations were taken into account in iterative extensions of the library. Synthesis and evaluation of the compounds in PPlase assays resulted in highly active inhibitors. The activities can be interpreted in terms of a common binding mode obtained by docking calculations. (C) 2016 Elsevier Ltd. All rights reserved.
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