Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1612, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2019.460647
Keywords
Aldehydes; Beer staling; HS-SPME-GC-MS; Pale malt; Sample preparation; Extraction parameters
Funding
- European Union's Horizon 2020 research and innovation programme under the Marie Skiodowska-Curie grant [722166]
Ask authors/readers for more resources
Aldehydes originating from malt play an important role in beer flavour deterioration. In order to better understand the influence of malting process on beer staling, it is necessary to acquire a reliable analytical methodology for determination of beer staling aldehydes in malt. Therefore, the aim of this study was to evaluate extraction parameters, which allow quantification of beer staling aldehydes present in pale malts. The method was validated with respect to linearity (R > 0.9988), limit of detection (0.28 - 0.99 mu g/L), limit of quantification (0.92 - 3.31 mu g/L), accuracy (+/- 5%), repeatability (1.3 - 5.3%) and intermediate precision (>20%). The following parameters of sample preparation were evaluated: sample amount, extraction time and temperature, ultrasonication time and oxygen level. Consequently, the best extraction conditions were successfully applied on pale malts. After extraction, the samples were analysed by headspace solid-phase microextraction (HS-SPME) with on fibre carbonyl derivatisation followed by gas chromatography and mass spectrometry (GC-MS). In addition, the salting-out effect during HS-SPME was studied. The method application allowed to identify significant differences (p <= 0.05) in the levels of aldehydes among various industrial scale, pale malts. The optimised method could give the information on the aldehyde content introduced into the brewing process and its potential contribution to the overall beer quality. (C) 2019 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available