Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 121, Issue 2, Pages 1911-1922Publisher
WILEY
DOI: 10.1002/jcb.29426
Keywords
gastric cancer; LPAR1; migration; miR-501-5p; proliferation
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Funding
- Medical Science and Technology Development Foundation, Nanjing Department of Health [YKK16224, YKK16233, YKK17208]
- Youth talent support program of Nanjing City [QRX17107, QRX17208]
- Jiangsu Provincial Commission of Health and Family Planning [Z201603]
- Natural Science Foundation of Jiangsu Province [BK20171510]
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In spite of the achievement in treatment, the gastric cancer (GC) mortality still remains high. MicroRNAs (miRNAs) are a group of small noncoding RNAs that play a crucial part in tumor progression. In this study, we explored the expression and function of microRNA-501-5p (miR-501-5p) in GC cell lines. Quantitative real-time polymerase chain reaction assay results suggested that miR-501-5p was significantly upregulated in GC tissues and cell lines. And, the Cell Counting Kit-8 colony formation and cell migration assay results showed that the downregulation of miR-501-5p decreased GC cell proliferation and migration. Besides that, we found that GC cell cycle was arrested in G2 phase and cell apoptosis rate was increased by silencing the expression of miR-501-5p in GC cell lines using the flow cytometry. We also found that miR-501-5p could directly target lysophosphatidic acid receptor 1 (LPAR1) and negatively regulate LPAR1 expression in GC cell lines by performing dual-luciferase reporter gene assay and Western blot analysis. And, LPAR1 was significantly downregulated in GC tissues and inversely correlated with miR-501-5p expression. Furthermore, LPAR1 downregulation promoted cell proliferation and migration, which were attenuated by cotransfection of miR-501-5p inhibitor in GC cells. In conclusion, miR-501-5p can promote GC cell proliferation and migration by targeting and downregulating LPAR1. miR-501-5p/LPAR1 may become a potential therapeutic target for GC treatment.
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