Journal
JOURNAL OF CELL BIOLOGY
Volume 218, Issue 11, Pages 3560-3571Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201901108
Keywords
-
Categories
Funding
- Department of Science and Technology (India) INSPIRE Faculty Award
- Department of Biotechnology (India) Innovative Young Biotechnologist Award
- Science and Engineering Research Board (India) Early Career Research Award
- Wellcome Trust/Department of Biotechnology-India Alliance Intermediate Fellowship [IA/18/1/503607]
Ask authors/readers for more resources
During sexual reproduction in eukaryotes, processes such as active degradation and dilution of paternal mitochondria ensure maternal mitochondrial inheritance. In the isogamous organism fission yeast, we employed high-resolution fluorescence microscopy to visualize mitochondrial inheritance during meiosis by differentially labeling mitochondria of the two parental cells. Remarkably, mitochondria, and thereby mitochondrial DNA from the parental cells, did not mix upon zygote formation but remained segregated at the poles by attaching to clusters of the anchor protein Mcp5 via its coiled-coil domain. We observed that this tethering of parental mitochondria to the poles results in uniparental inheritance of mitochondria, wherein two of the four spores formed subsequently contained mitochondria from one parent and the other spores contained mitochondria from the other parent. Further, the presence of dynein on an Mcp5 cluster precluded the attachment of mitochondria to the same cluster. Taken together, we reveal a distinct mechanism that achieves uniparental inheritance by segregation of parental mitochondria.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available