Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 295, Issue 1, Pages 69-82Publisher
ELSEVIER
DOI: 10.1074/jbc.RA119.009548
Keywords
cardiovascular disease; cell death; glutathione peroxidase; iron; ischemia; transcription repressor; (BTB domain and CNC homolog 1) BACH1; ferroptosis; iron sequestration; myocardial infarction
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Funding
- Japan Society for the Promotion of Science [19K07680, 16K07108, 15H02506, 24390066, 21249014, 18H04021]
- Agency for Medical Research and Development [JP16gm050001]
- Grants-in-Aid for Scientific Research [19K07680, 24390066, 18H04021, 21249014, 16K07108] Funding Source: KAKEN
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Ferroptosis is an iron-dependent programmed cell death event, whose regulation and physiological significance remain to be elucidated. Analyzing transcriptional responses of mouse embryonic fibroblasts exposed to the ferroptosis inducer erastin, here we found that a set of genes related to oxidative stress protection is induced upon ferroptosis. We considered that up-regulation of these genes attenuates ferroptosis induction and found that the transcription factor BTB domain and CNC homolog 1 (BACH1), a regulator in heme and iron metabolism, promotes ferroptosis by repressing the transcription of a subset of the erastin-induced protective genes. We noted that these genes are involved in the synthesis of GSH or metabolism of intracellular labile iron and include glutamate-cysteine ligase modifier subunit (Gclm), solute carrier family 7 member 11 (Slc7a11), ferritin heavy chain 1 (Fth1), ferritin light chain 1 (Ftl1), and solute carrier family 40 member 1 (Slc40a1). Ferroptosis has also been previously shown to induce cardiomyopathy, and here we observed that Bach1(?/?) mice are more resistant to myocardial infarction than WT mice and that the severity of ischemic injury is decreased by the iron-chelator deferasirox, which suppressed ferroptosis. Our findings suggest that BACH1 represses genes that combat labile iron-induced oxidative stress, and ferroptosis is stimulated at the transcriptional level by BACH1 upon disruption of the balance between the transcriptional induction of protective genes and accumulation of iron-mediated damage. We propose that BACH1 controls the threshold of ferroptosis induction and may represent a therapeutic target for alleviating ferroptosis-related diseases, including myocardial infarction.
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