Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 32, Issue 1, Pages 553-561Publisher
SPRINGER
DOI: 10.1007/s10811-019-01947-x
Keywords
Rhodophyta; Gracilaria gracilis; Phycobiliprotein; R-phycoerythrin; Purification; Gel filtration; Anion-exchange chromatography
Funding
- Funds for Science and Technology Development of the University of Danang, Vietnam [B2018-DN06-14]
- Vietnam Ministry of Education Training
- University of Danang
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Gracilaria gracilis, a red macroalga, represents an exploited important biomass. One of the potential uses of this red seaweed is the production of valuable molecules such as R-phycoerythrin. This compound has recently found applications in food and cosmetic industries as a natural colorant, in conducting medical diagnosis and in biology. However, after R-phycoerythrin extraction the protein extract has to be concentrated and pre-purified, which is commonly expensive and requires many steps. In this paper, R-phycoerythrin from the crude extract obtained by phosphate buffer 20 mM, pH 7.1, was purified by one-step chromatographic method. Native R-phycoerythrin was achieved with a high purity index (A(565)/A(280) ratio) of 3.25 (3.2 = standard R-phycoerythrin purity) after purification on DEAE Sepharose fast flow chromatography with the obtained fraction at 200 mM NaCl. This fraction presented the absorption and emission spectra of R-phycoerythrin with major absorbance at 498, 540, and 565 nm. Native form of this molecule was presented about 260 kDa. Besides, the purified pigment R-phycoerythrin included four subunits: alpha subunit (18 kDa), beta subunit (21 kDa), gamma subunit (29 kDa), and gamma' subunit (27 kDa) by gel electrophoresis.
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